Hilo stands for highly inclined and laminated optical sheet but is affectionately referred to as dirty tirf due to the slightly lower snr in comparison to tirf imaging. Highly inclined and laminated optical sheet hilo microscopy is an optical technique that employs a highly inclined laser beam to illuminate the sample with a thin sheet of light that can be scanned through the sample volume 1 hilo is an efficient illumination technique when applied to fluorescence imaging of thick samples owing to the confined illumination volume that allows high. In this study we developed simple easy to use microfluidic device for highly inclined and laminated optical sheet hilo microscopy using a model plant physcomitrella patens.
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